Avtor/Urednik		Ansaldi, Mireille; Marolt, Darja; Štebe, Tina; Mandič-Mulec, Ines; Dubnau, D
Naslov		Specific activation of the Bacillus quorum-sensing systems by isoprenylated pheromone variants
Tip		članek
Vir		Mol Microbiol
Vol. in št.		Letnik 44, št. 6
Leto izdaje		2002
Obseg		str. 1561-73
Jezik		eng
Abstrakt		Natural genetic competence in Bacillus subtilis is controlled by quorum-sensing (QS). The ComP- ComA two-component system detects the signalling molecule ComX, and this signal is transduced by a conserved phosphotransfer mechanism. ComX is synthesized as an inactive precursor and is then cleaved and modified by ComQ before export to the extracellular environment. The comQXP' loci of a set of natural Bacillus isolates have been sequenced and shown to possess a striking polymorphism that determines specific patterns of both activation and inhibition of the quorum-sensing response. We have developed a simple purification method for the modified peptide signalling pheromones allowing the characterization of four distinct ComX molecules representing different pherotypes. Genetic and biochemical evidence demonstrate that all the ComX variants are isoprenylated by the post-translational modification of a conserved tryptophan residue and that the modifications on the ComX peptide backbones vary in mass among the various pherotypes. These results give new insights into peptidemediated quorum-sensing signalling in Gram-positive bacteria and emphasize the role of isoprenylation in bacterial signal transduction.
Deskriptorji		BACILLUS SUBTILIS BACTERIAL PROTEINS PHEROMONES SIGNAL TRANSDUCTION SPECIES SPECIFICITY PROTEIN PROCESSING, POST-TRANSLATIONAL PROTEIN ISOPRENYLATION ESCHERICHIA COLI PEPTIDE SYNTHETASES PHYLOGENY POLYMORPHISM (GENETICS)