Author/Editor		Ivankovič, Dušica; Pavičić, Jasenka; Raspor, Biserka; Falnoga, Ingrid; Tušek-Žnidarič, Magda
Title		Comparison of two SH-based methods for estimation of metallothionein level in the digestive gland of naturally occuring mussels, Mytilus galloprovincialis
Type		članek
Source		Int J Environ Anal Chem
Vol. and No.		Letnik 83, št. 3
Publication year		2003
Volume		str. 219-31
Language		eng
Abstract		Two methods for the evaluation of metalothionein level in Mytilus galloprovincialis digestive gland homogenate extract, polarographic and spectrophotometric, were compared in combination with sample subjected to heat treatment (at 70 and 85 degrees C) as well to solvent precipitation. Spectrophotometric measurements ranging within 0.0181-0.144 mg MTg(-1) were at least one order of magnitude lower in comparison with the polarographic ones (2.21-2.88 mg MTg(-1)) depending on the particular sample treatment as well as two specific reagens and reference standards applied. Sephadex G-75 column chromatography of the differently trated samples and subsequent polarographic analysis in the pooled elution fractions indicated that a level of nonMT interfering thiolic component in homogenate extract may be accounted for more than 50% of the total polarographic signal, irrespetive of a method of sample treatment applied. Consequently, the large discrepancy between methods could not be attributed only to the sample treatment procedures but more likely to certain not entirely explained analytical problems, particulary calibration with appropriate reference standard.
Descriptors		METALLOTHIONEIN MUSSELS DIGESTIVE SYSTEM SHELLFISH SEAWATER WATER POLLUTANTS CHROMATOGRAPHY, GEL POLAROGRAPHY SPECTROPHOTOMETRY