Avtor/Urednik		Gabrijel, Mateja; Repnik, Urška; Kreft, Marko; Grilc, Sonja; Jeras, Matjaž; Zorec, Robert
Naslov		Quantification of cell hybridoma yields with confocal microscopy nad flow cytometry
Tip		članek
Vir		Biochem Biophys Res Commun
Vol. in št.		Letnik 314
Leto izdaje		2004
Obseg		str. 717-23
Jezik		eng
Abstrakt		The fusion of antigen presenting and cancer cells leads to the formation of hybrid cells, which are considered a potential vaccine for treating cancer. The quality assessment of hybrid cell vaccines is crucial for the introduction of this new treatment. Flow cytometry was the method used recently, since it is faster in comparison to classical microscopy. Here we describe a rapid confocal microscopy based approach to quantify hybrid cell yields. The extent of fusion rate was determined by confocal microscopy by counting dual fluorescent cells and by measuring the area of co-localized pixels. Results of both methods showed high degree of correlation. The same samples were also analyzed by flow cytometry. Fusion rates determined with both techniques showed significant correlation. In conclusion, using confocal microscopy we developed a sensitive and a rapid method to assess the yield of hybridomas in a large number of electrofused cells.
Deskriptorji		HYBRIDOMAS PHEOCHROMOCYTOMA DENDRITIC CELLS MICROSCOPY, CONFOCAL FLOW CYTOMETRY