Author/Editor		Gruden, Kristina
Title		Molekularno-biološke tehnike ugotavljanja prisotnosti gensko spremenjenih organizmov
Type		članek
Source		In: Raspor P, editor. Mikrobiologija in biotehnologija v proizvodnji živil. Ljubljana: Biotehniška fakulteta,
Publication year		2004
Volume		str. 171-7
Language		slo
Abstract		Using molecular biology techniques we can detect transgenic plants DNA in samples. As the DNA is quite stable, we can use this approach for detection of transgenic plants in different samples, crops, seeds, feed and food. Basic methods used in detection of GMOs are PCR and Real-time PCR. PCR is used when qualitative analysis is performed, while Real-time PCR is performed for quantitative analysis, that is determination of percentage of GMO considering the amount of the certain plant species in the sample. DNA, inserted into the plant, is composed of the gene coding for the specific property surrounded by regulatory regions, which regulate expression of desired property in the plant. Regulatory regions can be the same in different transgenic plants, while genes, wding for certain property, are specific for certain GMO. If we perform PCR for detection of regulatory regions, we can screen the sample for many transgenic plants at the same time. On the other hand, if we perform PCR amplification of the gene inserted, we analyse the sample for that GMO only. For quantitative analysis we determine concentration of transgene in the sample and wncenlration of plant specific DNA in parallel using Real time PCR. For correct results it is necessary to adequately perform all steps in the procedure - sampling, DNA isolation and qualitative and quantitative analysis, respectively.
Descriptors		GENETIC ENGINEERING POLYMERASE CHAIN REACTION DNA, RECOMBINANT PLANTS, TRANSGENIC