Avtor/Urednik		Čučnik, S; Kveder, T; Križaj, I; Rozman, B; Božič, B
Naslov		High avidity anti-beta2-glycoprotein I antibodies in patients with antiphospholipid syndrome
Tip		članek
Vir		Ann Rheum Dis
Vol. in št.		Letnik 63, št. 11
Leto izdaje		2004
Obseg		str. 1478-82
Jezik		eng
Abstrakt		Objective: To evaluate avidity of IgG anti-beta2-glycoprotein I antibodies (anti-beta2-GPI) in patients with antiphospholipid syndrome (APS) and systemic lupus erythematosus (SLE) in relation to thrombosis, and to demonstrate a possible affinity maturation of IgG anti-beta2-GPI during the disease course. Methods: 64 sera from 32 patients (18 with primary or secondary APS, 14 with SLE without APS) and their respecfive IgG fractions or affinity purified anti-beta2-GPI were studied by anticardiolipin (aCL) and anti-beta2GPI enzyme linked immunosorbent assay and by chaotropic assay. Results: Six, 12, and 14 patients had high, low, and heterogeneous avidity IgG anti-beta2-GPI, respectively. In 12 patients an increase in antibody avidity was observed over a period of between four and 12 years. More patients with APS were in the high avidity than in the low avidity anti-beta2-GPI group, while the opposite was observed for SLE alone (both p<.0.05). The most common clinical feature among patients with high avidity anti-beta2-GPI was thrombosis, mainly venous thrombosis (p<0.05 and p<0.02, respectively, v the low avidity anti-[beta2-GPI group). Conclusions: Patients with APS with or without SLE may have anti-betai2-GPI of high, low, or heterogeneous avidity. High avidity anti-beta2-GPI appear to be associated with thrombosis and APS, while in pure SLE low avidity anti-beta2-GPI may prevail. Monitoring of avidity may help elucidate the role of anti-beta2-GPI affinity maturation in the pathogenesis of APS.
Deskriptorji		ANTIPHOSPHOLIPID SYNDROME LUPUS ERYTHEMATOSUS, SYSTEMIC ANTIBODIES, ANTICARDIOLIPIN IGG AUTOANTIBODIES ANTIBODIES, ANTINUCLEAR ENZYME-LINKED IMMUNOSORBENT ASSAY FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT