Author/Editor		Glavač, Damjan; Berginc, Gašper; Luzar, Boštjan; Poljak, Mario; Ferlan-Marolt, Vera
Title		Microsatellite instability in hepatitis C virus infected patients
Type		članek
Source		In: Ferlan-Marolt V, Luzar B, editors. Viral hepatitis. Proceedings of the 35th memorial meeting to professor Janez Plečnik with international participation; 2004 Dec 2-3; Ljubljana. Ljubljana: Faculty of medicine, Institute of pathology,
Publication year		2004
Volume		str. 93-7
Language		eng
Abstract		Background: Hepatitis C virus (HCV) core protein of 1b and 2a HCV genotypes has been shown to promote microsatellite instability (MSI) on human non-neoplastic hepatocytes and thereby disturb the DNA mismatch repair (MMR) system. To investigate the influence of different HCV genotypes on the DNA MMR system, we studied 26 HCV infected patients for MSI and LOH using five selected microsatellite markers in fluorescence based PCR and semi-automated laser scanning fragment analysis. Patients and methods: DNA was extracted from liver tissue obtained by percutaneous liver biopsy and from peripheral blood at the same time as serum was collected for genotypization. DNA was amplified in PCR with 5 microsatellite fluorescently labelled markers (BAT 25, BAT 26, BAT 40, D18S58 and D17S250) and analyzed in an ABI PRISM 310 Genetic Analyzer. A sample was defined as high-MSI (MSI-H) if more than one of the 5 examined loci showed uneguivocal instabilities. Results: High MSI was detected in 5/11 (46%) of HCV patients with genotype 1b highMSI and in 0/15 HCV patients with genotypes 1a, 2b or 3 (p=0.021). Conclusions: The high-MSI found in 46% of HCV patients with genotype 1b and with histologically proven early stage of liver cirrhosis indicates important differences among HCV genotypes in relation to DNA MMR system. It also indicates that high-MSI in HCV patients withgenotype 1b may be an early event and provide a possible molecular mechanism for the progression of chronic hepatitis to cirrhosis and liver cancer.
Descriptors		HEPATITIS C HEPATITIS C-LIKE VIRUSES GENOTYPE MICROSATELLITE REPEATS POLYMERASE CHAIN REACTION