Author/Editor		Trinkaus, Miha
Title		Katepsina B in L ter njuna inhibitorja stefin V in cistatin C pri napredovanju intrakranialnih tumorjev
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2005
Volume		str. 164
Language		slo
Abstract		Cathepsins B and L are lysosomal cysteine proteases implicated in progression of cancer. Elevation of cysteine proteases together with other types of endoproteases accelerates the invasion and metastasis of tumour cells. Intracellular control over the cysteine cathepsins' activity is theoretically disturbed also due to a downregulation of cystatins in relation to cysteine cathepsins, contributing to the malignant phenotype of cancer cells. In the present study we aimed to reveal the role of the cathepsin B in invasion of most abundant intracranial tumours, meningiomas and gliomas. In parallel, we wanted to reveal the possible role of cathepsins and their inhibitors as diagnostic and prognostic markers in these types of tumors. The first part of the study is dedicated to the analysis of meningiomas, whereas the second is dedicated to gliomas. Meningiomas are mostly benign tumours with long- term relapse, although they comprise also the histological similar subtypes, which are more aggressive and invasive. Among the more aggressive meningiomas, we can histologically characterise the type of atypical and anaplastic meningioma, with significantly shorter patients' survival. To better discriminate between the invasive meningioma subtypes in all three groups of meningiomas, we need more biological markers, among which are also cathepsins B and L and their endogenous inhibitors, studied in this work, both in vitro and in vivo. Using the assay of the overgrowth of primary tumour spheroids in vitro, we were able to show within a group of 3 benign meningiomas the more invasive tumour, which characteristically overgrew the normal tissue and where the invading cells stained intensively for cathepsin B in the pseudopodia. Cathepsins B was also observed in the migrating macrophages, which invaded the normal tissue. (Abstract truncated at 2000 characters).
Descriptors		CATHEPSINS CYSTEINE PROTEINASE INHIBITORS MENINGIOMA MENINGEAL NEOPLASMS ASTROCYTOMA NEOPLASM INVASIVENESS IMMUNOHISTOCHEMISTRY APOPTOSIS POLYMERASE CHAIN REACTION TRANSFECTION RECEPTORS, PROGESTERONE BLOTTING, WESTERN SURVIVAL ANALYSIS