Author/Editor     Mencinger, Marina; Aman, P
Title     Characterization of TFG in mus musculus and Caenorhabditis elegans
Type     članek
Source     Biochem Biophys Res Commun
Vol. and No.     Letnik 257, št. 1
Publication year     1999
Volume     str. 67-73
Language     eng
Abstract     TFG was discovered as a fusion partner of NTRK1 in human papillary thyroid carcinoma. We assembled the mouse TFG cDNA from EST sequences and 5' end RACE product, identified full coding length TFG EST clones in pig (c17b07) and Schistosoma mansoni (SMNAS62), and analyzed the genomic structure of TFG in Caenorhabditis elegans (Y63D3A). The protein sequences of mouse, pig, and S. mansoni TFG are highly homologous to human TFG. The C. elegans sequence has diverged, but its predicted secondary structure is remarkably conserved. Human, mouse, and C. elegans TFG contain a putative trimeric N-terminal coiled-coil domain, glycosylation, myristylation, and phosphorylation sites, and SH2- and SH3-binding motifs. The SH2-binding motif is absent in C. elegans TFG. The expression of TFG does not vary among 7, 11, 15, and 19 day mouse embryonal stages. In situ hybridization with a TFG probe in 10, 5-day whole mouse embryos showed preferential staining of the limb buds, branchial arches, nasal processes, and brain, and weak staining of the primitive spinal cord and dorsal root ganglia.
Descriptors     CAENORHABDITIS ELEGANS
GENE EXPRESSION REGULATION, DEVELOPMENTAL
GENES, HELMINTH
PROTEINS
PROTEIN STRUCTURE, SECONDARY
MICE
AMINO ACID SEQUENCE
BASE SEQUENCE
CHROMOSOME MAPPING
DNA, COMPLEMENTARY
EMBRYO
GLYCOSYLATION
MOLECULAR SEQUENCE DATA
RNA, MESSENGER
SCHISTOSOMIASIS MANSONI
SEQUENCE HOMOLOGY, AMINO ACID
SWINE
SRC HOMOLOGY DOMAINS