Author/Editor		Drobne, D; Milani, M; Lašer, V; Štrus, J
Title		Focused ion beam (FIB) / SEM system for in situ manipulation and microscopy of crustacean digestive system (Isopoda, Crustacea)
Type		članek
Source		In: Čeh M, Dražič G, Fidler S, editors. MCM 2005. Proceedings of the 7th multinational congress on microscopy; 2005 Jun 26-30; Portorož. Ljubljana: Slovene society for microscopy,
Publication year		2005
Volume		str. 111-4
Language		eng
Abstract		The focused ion beam (FIB) / SEM system was used to prepare cross-sections of selected regions of digestive gland epithelium of Porcellio scaber (Isopoda, Crustacea) for scanning electron microscopy. Application of a FIB / SEM system for morphological studies has advantages over conventional microscopy techniques because of: in situ site-specific 2D-sectioning and imaging of subsurface microstructures; no need to embed the sample prior to sectioning; and a possibility of using a wide range of magnifications while imaging the same sample. The FIB / SEM system is in the early stage of application in life sciences and appears to be a technique of choice for 3D-observations at the organism, organ, tissue and (sub)cellular levels.
Descriptors		CRUSTACEA DIGESTIVE SYSTEM MICROSCOPY, ELECTRON, SCANNING