Avtor/Urednik		Popova, Ljubomira; Hadjidekova, Valeria; Karadjov, Georgy; Agova, Savina; Traskov, Danail; Hadjidekov, Vassil
Naslov		Cytogenetic analysis of peripheral blood lymphocytes after arteriography (exposure to x-rays and contrast medium)
Prevedeni naslov		Citogenetska analiza limfocitov v periferni krvi po kontrastni arteriografiji
Tip		članek
Vir		Radiol Oncol
Vol. in št.		Letnik 39, št. 2
Leto izdaje		2005
Obseg		str. 153-8
Jezik		eng
Abstrakt		Backgrounds. The purpose of our study is to investigate the cytogenetic analysis findings in peripheral blood lymphocytes of 29 patients who had undergone diagnostic radiography. Methods. Peripheral blood samples were taken from 22 patients submitted to renal arteriography and 7 patients subnutted to cerebral arteriography (17 male and 12 female, aged between 13-68 years). Cytogenetic analyses of peripheral lymphocytes were performed before the procedure, immediately after and 24 hours later. The entrance skin dose obtained during the whole diagnostic X-ray exposure was measured by thermoluminescent dosimeters and varied between 0.03-0.30 Gy. Both low and high osmolarity contrast media were used. Chromosomal aberrations and micronuclei frequency were used as biomarkers of genotoxicity. Results. The estimated frequency of chromosomal aberrations and micronuclei in the peripheral blood lymphocytes of patients after arteriography examination was significantly higher than the level before the diagnostic exposure. The mean frequency of cells with chromosomal aberrations was nearly double after examination and proved to be constant in the analysis after 24 hours. Conclusions. Radiological diagnostic procedures involving iodinated contrast media as arteriography may cause a significant increase in cytogenetic damage in peripheral blood lymphocytes.
Izvleček		Izhodišča. Namen citogenetske raziskave je bil ugotoviti učinek diagnostične arteriografije na limfocite periferne krvi pri 29 bolnikih. Metode. Periferne vzorce krvi smo odvzeli 22 bolnikom, ki so bili napoteni na ledvično arteriografijo in 7 bolnikom napotenim na možgansko arteriografijo (17 moškim in 12 ženskam, starim 13 - 68 let). Citogenetska analiza limfocitov periferne krvi je bila narejena pred preiskavo, neposredno po preiskavi in 24 ur kasneje. Vstopno kožno dozo sevanja med celotno rentgensko preiskavo smo merili s termoluminescentnim dozimetrom in je bila 0,03-0,30 Gy. Uporabili smo kontrastno sredstva z nizko in visoko osmolarnostjo. Genotoksičnost smo ocenjevali s pogostnostjo kromosomskih aberacij in mikronukleusov. Rezultati. Pogostnost kromosomskih aberacij in mikronukleusov v limfocitih periferne krvi je bila značilno višja po arteriografiji, kot pa jih je bilo pred njo. Število kromosomskih aberacij se je skoraj podvojilo in je po 24 urah ostalo nespremenjeno. Zaključki. Rentgenske preiskave z jodnim kontrastnim sredstvom, kot je arteriografija, lahko statistično značilno zvišajo število citogenetskih poškodb v limfocitih periferne krvi.
Deskriptorji		ANGIOGRAPHY LYMPHOCYTES CHROMOSOME ABERRATIONS MICRONUCLEUS TESTS