Avtor/Urednik		Petrovič, Uroš; Šribar, Jernej; Matis, Maja; Anderluh, Gregor; Peter-Katalinić, Jasna; Križaj, Igor; Gubenšek, Franc
Naslov		Ammodytoxin, a secretory phospholipase A2, inhibits G2 cell cycle arrest in yeast Saccharomyces cerevisiae
Tip		članek
Vir		Biochem J
Vol. in št.		Letnik 391, št. 2
Leto izdaje		2005
Obseg		str. 383-8
Jezik		eng
Abstrakt		Ammodytoxin (Atx), an sPLA(2) (secretory phospholipase A(2)), binds to gamma and epsilon isoforms of porcine 14-3-3 proteins in vitro. 14-3-3 proteins are evolutionarily conserved eukaryotic regulatory proteins involved in a variety of biological processes, including cell-cycle regulation. We have now shown that Atx binds to yeast 14-3-3 proteins with an affinity similar to that for the mammalian isoforms. Thus yeast Saccharomyces cerevisiae can be used as a model eukaryotic cell, which lacks endogenous phospholipases A(2), to assess the in vivo relevance of this interaction. Atx was expressed in yeast cells and shown to be biologically active inside the cells. It inhibited G(2) cell-cycle arrest in yeast, which is regulated by 14-3-3 proteins. Interference with the cell cycle indicates a possible mechanism by which sPLA(2)s are able to cause the opposing effects, proliferation and apoptosis, in mammalian cells.
Deskriptorji		VIPER VENOMS PHOSPHOLIPASES A SACCHAROMYCES CEREVISIAE CELL CYCLE PROTEINS CELL DIVISION CELL DEATH