Avtor/Urednik		Koković, Ira; Golouh, Rastko; Jančar, Janez; Zidar, Andreja; Komel, Radovan
Naslov		Importance of molecular analysis in diagnosis of lymphomas
Prevedeni naslov		Pomen molekularno bioloških preiskav v diagnostiki limfomov
Tip		članek
Vir		In: Luzar B, Poljak M, Glavač D, et al, editors. Molekularna diagnostika v medicini. Zbornik 15. spominsko srečanje akademika Janeza Milčinskega, 36. memorialni sestanek profesorja Janeza Plečnika, 1. srečanje Slovenskega društva za humano genetiko z mednarodno udeležbo; 2005 30 nov - 2 dec; Ljubljana. Ljubljana: Medicinska fakulteta,
Leto izdaje		2005
Obseg		str. 151-8
Jezik		eng
Abstrakt		Demonstration of clonality and detection of specific genetic abnormalities enable distinguishing between neoplastic lesionsand reactive processes and, thus, have important value in the diagnosis of lymphoid neoplasms. A clonal population of lymphoid cells can be detected by PCR amplification of the rearranged immunoglobulin heavy chain (IgH) and T-cell receptor gama chain (TcRgama) genes. Similarly, the t(11;14)(q13;q32) in mantle cell lymphoma and the t(14;18)(q32;q21) in follicular lymphoma can be detected by PCR amplification of rearranged bcl-1/IgH and IgH/bcl-2 regions, respectively. We have introduced PCR-based assays for clonality analysis as well as for the detection of t(11;14) and t(14;18) chromosome translocations. Assays are simple, fast and can be applied on small amounts of formalin-fixed, paraffin-embedded tissue. Detection rates of our PCR assays are in agreement with reported data. We have been using molecular techniques as additional methods in the diagnosis of lymphoproliferative disorders since 1997
Deskriptorji		LYMPHOMA POLYMERASE CHAIN REACTION