Avtor/Urednik		Petrovec, Miroslav
Naslov		Non-commercial methods in diagnostic molecular microbiology
Prevedeni naslov		Uporaba nekomercialnih metod v klinični mikrobiologiji
Tip		članek
Vir		In: Luzar B, Poljak M, Glavač D, et al, editors. Molekularna diagnostika v medicini. Zbornik 15. spominsko srečanje akademika Janeza Milčinskega, 36. memorialni sestanek profesorja Janeza Plečnika, 1. srečanje Slovenskega društva za humano genetiko z mednarodno udeležbo; 2005 30 nov - 2 dec; Ljubljana. Ljubljana: Medicinska fakulteta,
Leto izdaje		2005
Obseg		str. 349-55
Jezik		eng
Abstrakt		In the past decade, innovations in nucleic acid amplification techniques, especially polymerase chain reaction (PCR), have had a major impact on diagnostics in clinical microbiology laboratories. One of the biggest advantages in the recent years has been the invention of real time PCR method and instruments. This has become the unifying platform for the amplification and detection of nucleic acids in many laboratories. Horizontal transfer of newly developed assays has become streamlined and empowered laboratories to adopt quickly and reliably the new technology. However, commercially standardized assays are available for only a limited array of targets. The growing need for a sensitive and specific detection of pathogens in clinical samples has driven the development of in-house assembled PCR assays for many other microorganisms for which specific detection kits are not available. When a laboratory applies assembled PCR system in clinical diagnostics and the results are reported as such, the liability for verification of the performance characteristics rests on the laboratory. Guidelines for validation of assembeld PCR assays are being developed and every laboratory should implement them as a part of their rutine quality assurance.
Deskriptorji		POLYMERASE CHAIN REACTION ENCEPHALITIS