Author/Editor		Seme, Katja; Poljak, Mario
Title		Molecular diagnostics of bacterial pathogens
Translated title		Molekularna diagnostika medicinsko pomembnih bakterij
Type		članek
Source		In: Luzar B, Poljak M, Glavač D, et al, editors. Molekularna diagnostika v medicini. Zbornik 15. spominsko srečanje akademika Janeza Milčinskega, 36. memorialni sestanek profesorja Janeza Plečnika, 1. srečanje Slovenskega društva za humano genetiko z mednarodno udeležbo; 2005 30 nov - 2 dec; Ljubljana. Ljubljana: Medicinska fakulteta,
Publication year		2005
Volume		str. 393-400
Language		eng
Abstract		Molecular methods are at present mainly used for the molecular detection of bacteria directly in patients specimen, molecular detection and identification of new bacterial pathogens, molecular identification of bacterial pathogens grown in culture, molecular detection of antimicrobial resistance and characterization of bacterial pathogens beyond identification. Hybridization methods are highly specific for the detection and identifcation of bacterial pathogens, but limited by their sensitivity. The development of molecular amplification methods, not dependent on bacterial multiplication, has enabled a faster and more sensitive detection of target bacteria. Broad-range ribosomal polymerise chain reaction (PCR) and sequence-based analysis have played and still do play key roles in the characterization of navel bacterial pathogens, notably in the settings where the detection by culture has failed. The development of rapid molecular assays targeting specific antibiotic resistance determinants has improved the detection of bacterial pathogens with certain resistance mechanisms.
Descriptors		BACTERIAL INFECTIONS POLYMERASE CHAIN REACTION