Author/Editor		Keše, Darja
Title		Molecular diagnostics of chlamydia and mycoplasma infections
Translated title		Molekularna diagnostika klamidijskih in mikoplazemskih okužb
Type		članek
Source		In: Luzar B, Poljak M, Glavač D, et al, editors. Molekularna diagnostika v medicini. Zbornik 15. spominsko srečanje akademika Janeza Milčinskega, 36. memorialni sestanek profesorja Janeza Plečnika, 1. srečanje Slovenskega društva za humano genetiko z mednarodno udeležbo; 2005 30 nov - 2 dec; Ljubljana. Ljubljana: Medicinska fakulteta,
Publication year		2005
Volume		str. 409-16
Language		eng
Abstract		Molecular methods have shown to be of a great value for the detection of fastidious, slow-growing organisms such as chlamydiae and mycoplasma. Moreover, recently introduced molecular assays turned out to be more sensitive as culture methods and offer a better approach for the rapid detection of these pathogens. The broad spectrum of established and well evaluated commercial test systems for the molecular detection of the urogenital pathogen Chlamydia trachomatis has yet been created for the routine use in clinical microbiology laboratories. The major targets for the amplification based tests tar C. trachomatis are generally the cryptic chlamydial plasmid or rRNA. On the contrary, no standardized nucleic acid-based assay for detecting Chlamydia pneumoniae and Mycoplasma pneumonie has been created until recently. A great progress in the molecular diagnosis of C. pneumoniae and M pneumoniae infections has come out with the recently developed real-time PCR technology. It offers a rapid amplification and sequence-specific detection of amplicons in an automated and standardized format. Another advantage in the molecular diagnosis of these respiratory pathogens has been recently achieved with the development of multiplex real-time PCR to detect and discriminate the infections with C. pneumoniae, M. pneumoniae and Legionella spp. We can conclude that molecular diagnostic techniques which use PCR have proven to be an important tool for a rapid etiologic diagnosis of Chlamydia and Mycoplasma infections.
Descriptors		CHLAMYDIA INFECTIONS MYCOPLASMA INFECTIONS POLYMERASE CHAIN REACTION CHLAMYDIA TRACHOMATIS MYCOPLASMA PNEUMONIAE