Avtor/Urednik		Kojc, Nika; Zidar, Nina; Vodopivec, Boris; Gale, Nina
Naslov		Expression of CD34, alpha-smooth muscle actin, and transforming growth factor beta1 in squamous intraepithelial lesions and squamous cell carcinoma of the larynx and hypopharynx
Tip		članek
Vir		Hum Pathol
Vol. in št.		Letnik 36
Leto izdaje		2005
Obseg		str. 16-21
Jezik		eng
Abstrakt		Aim of the study: There is increasing evidence that reaction in cancer has an important diagnostic and prognostic significance. Recent studies have shown that CD34-positive stromal cells and myofibroblasts may play an important role in host response to invasive cancer. The aim of our study was to analyze the expression of CD34, alfa-smooth muscle actin (SMA), and transforming growth factor beta1 (TGFbeta1) in squamous intraepithelial lesions (SILs) and squamous cell carcinoma (SCC) of the larynx and hypopharynx, to establish their significance, and to elucidate the mechanism of myofibroblast formation. Methods. Immunohistochemistry was performed on samples of 42 resected larynges and 12 laryngeal biopsies of SILs and SCC using antibodies against SMA, CD34, CD31, TGFbeta1, and TGFbeta1 receptors. The expression of TGFbeta1 mRNA was detected with RNA in situ hybridization using specific oligonucleotides for TGFbeta1. Results: The stroma in normal laryngeal mucosa and SILs contained scattered CD34-positive cells, but there were no SMA-positive myofibroblasts. In contrast, the stroma of SCC contained SMA-positive myofibroblasts, but there were no CD34-positive stromal cells. This pattern of stromal reaction was also observed in the peritumoral zone. In adjecent normal tissue, there were CD34-positive stromal cells and no myofibroblasts. We found more intense TGFbeta1 expression in carcinoma cells than in the normal laryngeal epithelium and positive staining for both TGFbeta1 receptors on stromal cells of the normal mucosa. In SCC, many myofibroblasts expressed TGFbeta1 and both receptors for TGFbeta1. Expression of TGFbeta1 mRNA was similar to expression of TGFbeta1 protein. Conclusion: Our study shows that disappearance of CD34-positive stromal cells and appearance of SMA-positive stromal myofibroblasts are associated with transformation of laryngeal SILs to SCC. (Abstract truncated at 2000 characters)
Deskriptorji		LARYNGEAL NEOPLASMS CARCINOMA, SQUAMOUS CELL HYPOPHARYNX PRECANCEROUS CONDITIONS TUMOR MARKERS, BIOLOGICAL AGE FACTORS ACTINS SEX FACTORS IMMUNOHISTOCHEMISTRY IN SITU HYBRIDIZATION MUSCLE, SMOOTH RNA, MESSENGER TRANSFORMING GROWTH FACTOR BETA