Author/Editor		Brecelj, Erik
Title		Prognostični pomen klinično-patoloških značilnosti, izražanje E-kadherina, DNA ploidije in proliferacijske aktivnosti pri folikularnem karcinomu ščitnice
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2006
Volume		str. 92
Language		slo
Abstract		AlM: The aim of our study was to evaluate the expression of E-cadherin, DNA ploidy, and proliferative activity, as assessed by detemilnation ot S phase fraction (SPF) and Ki-67 expression, in follicular carcinoma of the thyroid and to correlate these findings with classical clinicopathological features of the tumors and with survival of the patients. MATERIAL AND METHODS: Patients who were treated at the Institute of Oncology Ljubljana between 1980 and 1995 and in whom pathologic reassessment of original slides confirmed the diagnosis ot follicular carcinoma or its variant (oncocytic or insular carcinoma) were included in the study. After exclusion ot 19 cases that showed histologic features of follicular variant ot papillary carcinoma, the tinal group consisted ot 70 patients (54 females, 16 males) whose age ranged kom 19 to 83 years (median, 63 years). The patients were followed fora median of 10.5 years.E-cadherin and Ki-67 expression was assessed immunohistochemicaily in sections from paraffin-embedded tumor tissues. Determination of DNA ploidy and SPF was pertormed by f]ow-cytometric examination ot cell suspensions obtained by disaggregation ot paratfin blocks. RESULTS: 10-year overal survival, cancer specific survival and local recurrence-tree survival were 56,8%, 68%, and 81,6%, respectively. Among 54 patients who had no distant metastasis at diagnosis, 10-year distant metastasis-tree survival was 73.3%.Reduced E-cadherin expression, defined as < 90% of cells showing membrane positivity, was tound in 24/70 (34%) tumors and was significantly associated with widely invasive growth, lesser degree of ditferentiation and presence of distant metastases at diagnosis. In univariate analysis patients with reduced E-cadherin had worse overall survival p=0,026), cancer-specific survival (p=0,OOl 3) and distant metastasis-free survival (p=0,0004).DNA ploidy and SPF could be assessed in 59 tumors. (Abstract truncated at 2000 characters).
Descriptors		THYROID NEOPLASMS CADHERINS PLOIDIES NEOPLASM METASTASIS NEOPLASM INVASIVENESS FLOW CYTOMETRY PROGNOSIS IMMUNOHISTOCHEMISTRY SURVIVAL ANALYSIS KI-67 ANTIGEN