Author/Editor		Domanovič, Dragoslav; Wozniak, Gordana; Černelč, Peter; Samardžija, Marina; Balen-Marunić, Sanja; Rožman, Primož
Title		Matrix metalloproteinase-9 and cell kinetics during the collection of peripheral blood stem cells by leukapheresis
Type		članek
Source		Transfusion and apheresis science
Vol. and No.		Letnik 33
Publication year		2005
Volume		str. 37-45
Language		eng
Abstract		The plasma levels of matrix metalloproteinase-9 (MMP-9) were determined during 41 collections of peripheral blood stem cells (PBSC) by standard volume (two blood volumes) leukaphereses (SVL) in 29 donors (7 allogeneic and 22 autologous) mobilized with the granulocyte-colony stimulating factor (G-CSF). The association between MMP-9 levels and cell counts in donor's blood was explored. During the processing of the first blood volume (BV), MMP-9 levels declined on average by 31% and persisted at the same level during the processing of the second BV. During the collection, a slight decline of white blood cells (WBC), polymorphonuclear neutrophils (PMN) and platelets (PLT) in donor's blood was accompanied by a significant drop of CD34+ cells by 37% after 1 BV and by 44% after 2 BV had been processed (p = 0.001). The drop of MMP-9 plasma levels showed a loose correlation with the decrease of WBC (r = 0.68, p = 0.002) and PMN counts (r = 0.67, p = 0.001). We conclude that the levels of MMP-9 that have been elevated by the mobilization of donors with G-CSF, decrease during the collection of PBSC by 4 h SVL. The observed decrease was indirectly related to the drop of WBC and PMN counts, suggesting that certain other factors have an influence on MMP-9 kinetics during PBSC collection.
Descriptors		HEMATOPOIETIC STEM CELLS LEUKAPHERESIS METALLOPROTEINASES BLOOD DONORS GRANULOCYTE COLONY STIMULATING FACTOR, RECOMBINANT BLOOD CELL COUNT FLOW CYTOMETRY