Avtor/Urednik		Bocos, C; Castro, M; Orozco, E; Contreras, JA; Herrera, E
Naslov		A rapid and sensitive method for HPLC cholesterol determination in bile
Tip		članek
Vir		Rev Esp Fisiol
Vol. in št.		Letnik 48, št. 3
Leto izdaje		1992
Obseg		str. 211-4
Jezik		eng
Abstrakt		A relatively little time consuming simple method based on the treatment of bile with cholesterol oxidase and subsequent high performance liquid chromatography measurement of the 3-ketocholesterol produced in order to determine the level of the cholesterol concentration is described. The method avoids bilirubin interferences, has high reproducibility and recovery assays give 100% values. It is highly sensitive and suitable for use in the determination of cholesterol concentrations in bile and other bilirubin containing biological fluids.
Deskriptorji		CHROMATOGRAPHY, HIGH PRESSURE LIQUID BILE CHOLESTEROL KETOCHOLESTEROLS RATS, SPRAGUE-DAWLEY REPRODUCIBILITY OF RESULTS SENSITIVITY AND SPECIFICITY TIME FACTORS