Avtor/Urednik		Sillanpaa, Heidi; Lahdenne, Pekka; Sarvas, Heikki; Arnež, Maja; Steere, Allen; Peltomaa, Miikka; Seppala, Ilkka
Naslov		Immune responses to borrelial VlsE IR6 peptide variants
Tip		članek
Vir		Int J Med Microbiol
Vol. in št.		Letnik 297, št. 1
Leto izdaje		2007
Obseg		str. 45-52
Jezik		eng
Abstrakt		Laboratory confirmation of Lyme borreliosis (LB) relies mainly on the demonstration of anti-borrelial antibodies. In recent studies, a novel VlsE protein IR(6) peptide-based assay has been introduced. Our aim was to evaluate the IR(6) peptides from three Borrelia burgdorferi sensu lato genospecies in the serodiagnosis of European and North American patients. Five VlsE protein IR(6) peptide variants representing sequences from B. burgdorferi sensu stricto, B. garinii, and B. afzelii were used as antigens in both IgG and IgM enzyme-linked immunosorbent assays (ELISA). Serum antibodies of 187 patients at different stages of LB from Europe and the United States were evaluated for serodiagnosis. For comparison samples were tested with one of the commercial IR(6) ELISAs. Three B. afzelii IR(6) variant peptides revealed antibodies that were concordant with each other. B. burgdorferi sensu stricto peptide antibodies mostly paralleled B. afzelii peptide antibodies, and positive values were also obtained in the majority of European sera. For several sera, B. garinii IR(6) peptide antibodies were discordant to B. afzelii peptide antibodies. The commercial IR(6) peptide antibody assay (C6 ELISA) results correlated better with B. burgdorferi sensu stricto IR(6) than with B. garinii IR(6) peptide IgG results, especially in sera from patients with facial palsy. Thus, antibody specificity to IR(6) peptides may vary according to the infecting Borrelia species. In some manifestations of the disease, C6 ELISA may not cover all LB cases. Evidently, the methodological aspects in ELISA design for peptide antibody measurements are important as well as the amino acids sequence of the antigen.
Deskriptorji		BACTERIAL PROTEINS IGG IGM ANTIBODIES, BACTERIAL ANTIGENS, BACTERIAL BACTERIAL PROTEINS BORRELIA BURGDORFERI ENZYME-LINKED IMMUNOSORBENT ASSAY EUROPE LIPOPROTEINS LYME DISEASE NORTH AMERICA PEPTIDES SENSITIVITY AND SPECIFICITY