Author/Editor		Grmek-Košnik, Irena
Title		Optimizacija jemanja in obdelave nadzornih brisov za odkrivanje kolonizacije z MRSA
Translated title		Optimization of sampling and processing of surveilance cultures for the detection of MRSA colonization
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2006
Volume		str. 131
Language		slo
Abstract		Early identification of methicillin-resistant Staphylococcus aureus (MRSA) carriers is a major component of a MRSA control program. An ideal screening method would quickly identify all patients with MRSA, give no false-negative results, have both sensitivity and negative predictive values of 100% and have a low cost. Two factors are important in achieving this goal: the use of optimal culture methods (identification and susceptibility tests), and the optimal selection of anatomical sites for culture. The cost and laboratory workload could be markedly reduced by processing multiple swabs from one person in one culture broth (specimen pooling). We evaluated the sensitivity for detecting MRSA and the grow rate of pooled swabs compared to individual processing. Because we are making MRSA sectors from all suspicious colonies, we were interested if the number of MRSA colonies in culture medium influences the identification rate. We studied the impact of mixed bacterial flora and interaction between bacteria on likelihood of isolation of MRSA from swab. 1254 swabs from 423 subjects (2-5 swabs per subject) were submitted for detection of MRSA. Swabs were suspended in 2 ml volumes of sterile Todd-Hewitt Broth and divided into two 1-ml aliquots. One aliquot of the suspension was processed as a single specimen, whereas the other aliquot was mixed (pooled) with other suspensions in which swabs of the same patient were suspended. In the second part of research we examined the mutual impacts of bacterium MRSA and comensal flora ( coagulase negative staphylococci, diphteroidi, E. coli) on generational time in standardised conditions and vitro. We measured also the correlation between the density of comensal bacteria ( E. coli) and growth of endemic strain MRSA. We also determined the threshold for detection of MRSA from swab with classical microbiological cultivation. (Abstract truncated at 2000 characters)
Descriptors		STAPHYLOCOCCAL INFECTIONS STAPHYLOCOCCUS AUREUS METHICILLIN RESISTANCE ANTIBIOSIS CARRIER STATE NASAL MUCOSA COLONY COUNT, MICROBIAL SPECIMEN HANDLING CULTURE MEDIA