Author/Editor		Manojkumar, Ramanunninair
Title		Real-time reverse transcription polymerase chain reaction techniques: usefulness as an animal viral disease diagnostic
Translated title		Metoda obratnega prepisovanja in verižne reakcije s polimerazo v realnem času: uporabnost v diagnostiki živalskih virusnih bolezni
Type		članek
Source		Slov Vet Res
Vol. and No.		Letnik 43, št. 4
Publication year		2006
Volume		str. 155-60
Language		eng
Abstract		Quick and early diagnosis of the causative agents is critical for countries which are enzootic to particular disease. In these diagnostics speed is paramount; Real-time Reverse Transcription PCR (rRT-PCR) is being utilized increasingly in novel clinical diagnostic assays in molecular biology. The combination of excellent sensitivity and specificity made this technique an alternative to cell culture and other laboratory testing methods for disease diagnosis. In this review, the usefulness or applications of rRT-PCR assays in the diagnosis of some of the important animal viral infections are summarized.
Summary		Hitro in pravočasno odkrivanje povzročiteljev bolezni je v državah z enzootijo za določene bolezni bistvenega pomena. Pri taki diagnostiki je hitrost odločilna in rRT-PCR (obratno prepisovanje in verižna reakcija s polimerazo v realnem času) se čedafje več uporablja kot sodoben test klinične diagnostike v molekularni biologiji. Zaradi odlične občutljivosti in specifičnosti predstavfja alternativo celičnim kulturam in drugim laboratorijskim testom za diagnostiko bolezni. V preglednem članku povzemamo uporabnost in aplikacije testov rRT-PCR v diagnostiki nekaterih pomembnih živalskih virusnih bolezni.
Descriptors		RNA VIRUSES VIRUS DISEASES RETROVIRIDAE INFECTIONS POLYMERASE CHAIN REACTION