Avtor/Urednik		Pretnar, G; Filipič, B; Golob, A; Škodič, A; Toth, S; Mecs, I; Suhar, A
Naslov		Electroinduction of interferon-like proteins
Tip		članek
Vir		Bioelectrochem Bioenerg
Vol. in št.		Letnik 320, št. 25
Leto izdaje		1991
Obseg		str. 183-93
Jezik		eng
Abstrakt		To investigate the phenomena of electroinduction, human leukocytes/lymphocytes were exposed to electric pulses with a frequency of 2 Hz, duration time of 0.5 micro s and voltage of 250 V, for a period of 30 min. After 18 h of incubation at 37 degrees C, the samples were collected and analyzed. The proteins found were very similar in their behaviour to interferons (antiviral activity against Herpes simplex virus type 1 in vitro, antiproliferative activity against transformed human cells, sensitivity to pH 2 and to a temperature of 56 degrees C), and very much alike in molar mass (45.000 D) to tumor necrosis factor (TNF). Their isoelectric point (4.3) showed a similarity to subtype IV of human alpha-interferon (IFN). In spite of the great similarity of the unknown proteins to cytokines, immuno blot tests with monoclonal antibodies against interferon alpha, acidolabile, gamma, TNF and interleukin 2 did not reveal their identity.
Deskriptorji		INTERFERONS ELECTRIC STIMULATION LEUKOCYTES CELLS, CULTURED