Avtor/Urednik		Križman, M; Virant-Klun, I; Prošek, M
Naslov		Determination of derivatized amino acids in human embryo culture media by gas chromatography
Tip		članek
Vir		J Chromatogr B Analyt Technol Biomed Life Sci
Vol. in št.		Letnik 858, št. 1-2
Leto izdaje		2007
Obseg		str. 292-5
Jezik		eng
Abstrakt		An adequate analytical method for determination of amino acids can provide a better insight in the metabolism of in vitro human embryo cultures, increasing the success rate of embryo implantation. Since individual amino acid amounts per embryo occur in the nanogram range, GC was the technique of choice, due to its inherent sensitivity and high sample throughput. Amino acids were analyzed as alkyl formate derivatives. The limits of detection (LOD) of all amino acids involved were in the sub-nmol range. The high risk of sample contamination proved to be the major analytical issue, but it could be overcome. For an extended method sensitivity, a simple preconcentration step could also be used.
Deskriptorji		EMBRYO AMINO ACIDS CULTURE MEDIA CHROMATOGRAPHY, GAS