Author/Editor		Borko, Črtomir
Title		Humoral immune response to bacteria Borrelia burgdorferi s.l. in dairy cattle after experimental exposure
Translated title		Humoralni imunski odziv goveda po poskusnem stiku z bakerijo Borrelia burgdorferi s.l.
Type		članek
Source		Slov Vet Res
Vol. and No.		Letnik 45, št. 1
Publication year		2008
Volume		str. 15-24
Language		eng
Abstract		To acquire insights into the humoral immunal response (HIR) of grazing diary cattle naturally infected with B. burgdorferi sensu lato (s.l.), an experimental group of 3 adult dairy cows was artificially infected with the bacteria. Three cows were intradermally (i.d.) infected with living B. garinii, and another was immunized with dead bacteria in Freund’s incomplete adjuvant. The taxonomic identity and clonality of the isolates were confirmed by PCR amplification of the intergenic spacer between the 5S and 23S rRNA genes (1). The immune responses of the polyclonal, IgM and IgG class of antibodies were monitored both with whole-cell sonicated IgG and IgM and polyclonal ELISA and with indirect immunofluorescent assays (IIF). The immune response of the three i.d. infected animals remained low, but was detectable and characteristic in course, whereas in the vaccinated animal the immune response was strong.
Descriptors		BORRELIA BURGDORFERI SERODIAGNOSIS IGM IGG ANTIBODY FORMATION POLYMERASE CHAIN REACTION ENZYME-LINKED IMMUNOSORBENT ASSAY