Avtor/Urednik		Veranič, Peter; Lokar, Maruša; Schuetz, Gerhard J; Weghuber, Julian; Wieser, Stefan; Haegerstrand, Henry; Kralj-Iglič, Veronika; Iglič, Aleš
Naslov		Different types of cell-to-cell connections mediated by nanotubular structures
Tip		članek
Vir		Biophys J
Vol. in št.		Letnik 95, št. 9
Leto izdaje		2008
Obseg		str. 4416-4425
Jezik		eng
Abstrakt		Communication between cells is crucial for proper functioning of multi-cellular organisms. The recently discovered membranous tubes, named tunnelling nanotubes (TNTs), that directly bridge neighbouring cells, may offer a very specific and effective way of intercellular communication. Our experiments on RT4 and T24 urothelial cell lines show that nanotubes that bridge neighbouring cells can be divided into two types. The nanotubes of type I are shorter, more dynamic and contain actin filaments. They are formed when cells explore their surroundings in order to make contact with another cell. The nanotubes of type II are longer, more stable and have cytokeratin filaments. They are formed when two already connected cells start to move apart. On the nanotubes of both types small vesicles were found as an integral part of the nanotubes (i.e. dilatations of the nanotubes). The dilatations of type II nanotubes do not move along the nanotubes, while the nanotubes of type I have frequently dilatations (gondolas) that move along the nanotubes in both directions. A possible model of formation and mechanical stability of nanotubes that bridge two neighbouring cells is discussed.
Deskriptorji		CELL COMMUNICATION INTERCELLULAR JUNCTIONS UROTHELIUM CYTOSKELETON CELL LINE FLUORESCENT ANTIBODY TECHNIQUE MICROSCOPY, FLUORESCENCE MICROSCOPY, ELECTRON LIPOSOMES