Avtor/Urednik		Špirek, M; Horvath, A; Piškur, J; Sulo, P
Naslov		Functional co-operation between the nuclei of Saccharomyces cerevisiae and mitochondria from other yeast species
Tip		članek
Vir		Curr Genet
Vol. in št.		Letnik 38, št. 4
Leto izdaje		2000
Obseg		str. 202-7
Jezik		eng
Abstrakt		We elaborated a simple method that allows the transfer of mitochondria from collection yeasts to Saccharomyces cerevisiae. Protoplasts prepared from different yeasts were fused to the protoplasts of the ade2-1, ura3-52, kar1-1, rho0 strain of S. cerevisiae and were selected for respiring cybrids on plates containing 5-fluoroorotic acid and a non-fermentable carbon source. The identity of putative cybrids was assessed by restriction analysis of mitochondrial DNA, pulse field electrophoresis and tetrad analysis. In the comprehensive screening, only mitochondrial genomes from synonymous species (S. italicus, S. oviformis, S. capensis and S. chevalieri) exhibited complete compatibility with S. cerevisiae nuclei. The closely related S. douglasii mitochondrial genome could also partially restore respiration-deficiency in rho0 S. cerevisiae, whereas mitochondrial genomes from phylogenetically less related species could not.
Deskriptorji		CELL NUCLEUS DNA, MITOCHONDRIAL MITOCHONDRIA SACCHAROMYCES CEREVISIAE SPECIES SPECIFICITY YEASTS