Avtor/Urednik		Piškur, Jure
Naslov		Respiratory-competent yeast mitochondrial DNAs generated by deleting intergenic regions
Tip		članek
Vir		Gene
Vol. in št.		Letnik 81, št. 1
Leto izdaje		1989
Obseg		str. 165-8
Jezik		eng
Abstrakt		Two respiratory-competent yeast strains having mitochondrial (mt) DNA characterized by single non-overlapping deletions, encompassing intergenic sequences, have been crossed. Diploid daughter clones have been screened by electrophoresis of mtDNA fragments, and a respiratory-competent clone (ER8.75), having a recombinant small mtDNA with both parental deletions, has been detected. ER8.75 mtDNA lacks around 20% of wild-type intergenic sequences, encompassing three ori/rep sequences. This mutant could be helpful in analyzing the organelle genome and, particularly, the function of intergenic sequences.
Deskriptorji		CHROMOSOME DELETION CROSSES, GENETIC DNA RESTRICTION ENZYMES DNA, MITOCHONDRIAL SACCHAROMYCES CEREVISIAE