Author/Editor		Keše, Darja; Gubina, Marija; Kogoj, Rok; Strašek, Katja; Tepeš, Bojan
Title		Detection of point mutations in the gyrA gene of Helicobacter pylori isolates in Slovenia
Type		članek
Source		Hepatogastroenterology
Vol. and No.		Letnik 56
Publication year		2009
Volume		str. 925-9
Language		eng
Abstract		Backgrouns/aims The prevalence of antibiotic-resistant H. pylori clinical isolates is a growing concern. So far, fluoroquinolones have not been used to treat H. pylori on a large scale, but recent studies have reported a high rate of quinolones-resistance in H. pylori too. The aim of our study was to asses the mechanism of resistance to ciprofloxacin in H. pylori clinical isolates from patients living in Slovenia. Methodology: Out of 397 H. pylori clinical isolates, obtained in the period 1997 to 2004, 33 (8.3%) ciprofloxacin-resistant H. pylori isolates were recognized. DNA sequences of the gyrA gene were determined and translated into amino acid sequences. Results: Based on the results of this analysis, various point mutations in the ciprofloxacin-resistant clinical isolates were revealed. The most common mutations in H. pylori gyrA gene were found at codons corresponding to Asp91 (57.6%) and Asn87 (36.4%). Sequence analysis revealed amino acid substitutions also at codons Ala97 to Val, Ala129 to Thr and a double substitution at Asn87 to Lys and Val107 to Ile. Conclusion: These results suggest that H. pylori resistance to ciprofloxacin is already present in the Slovenian population and that it seems to be mediated through amino acid substitutions in the gyrA gene. Furthermore, the results obtained from the study also demonstrate no significant association between the type of gyrA mutation and the ciprofloxacin MIC level.
Descriptors		HELICOBACTER INFECTIONS HELICOBACTER PYLORI POINT MUTATION CIPROFLOXACIN DRUG RESISTANCE, MICROBIAL BASE SEQUENCE MICROBIAL SENSITIVITY TESTS GENOTYPE POLYMERASE CHAIN REACTION