| Author/Editor | Seme, Katja; Žnidovec-Lepej, Snježana; Lunar, Maja M; Iščić-Beš, Janja; Planinic, Ana; Kocjan, Boštjan J; Vince, Adriana; Poljak, Mario | |
| Title | Digene HPV genotyping RH test RUO: comparative evaluation with INNO-LiPA HPV genotyping extra test for detection of 18 high-risk and probable high-risk human papillomavirus genotypes | |
| Type | članek | |
| Source | J Clin Virol | |
| Vol. and No. | Letnik 46 | |
| Publication year | 2009 | |
| Volume | str. 176-9 | |
| Language | eng | |
| Abstract | Background: Standardized and validated methods for the specific detection and identification of a spectrum of high-risk (hr) HPV genotypes will be necessary if HPV genotyping gains an important role in the clinical management of HPV-related precancerous lesions and cancers. Objectives: The first comparative evaluation of novel HPV genotyping Digene HPV Genotyping RH Test RUO (Qiagen, Hilden, Germany) with standard INNO-LiPA HPV Genotyping Extra CE assay (Innogenetics, Gent, Belgium). Sudy design: Seventy hr-HPV positive samples were tested in parallel with both genotyping assays. The results were interpreted taking into account 15 hr-HPV and 3 probable hr-HPV genotypes that can be identified by both assays (assay-common genotypes). Results: Concordant results (a complete match of assay-common genotypes or negative using both assays) and compatible results (at least one genotype in common) were obtained in 42 (60.0%) and 28 (40.0%) samples, respectively. No discordant results for assay-common genotypes were obtained. Of 42 samples with compatible results, the presence of at least one assay-common genotype was detected in 37 samples, while no HPV was detected in two samples by both assays and only a single low-risk HPV was detected by INNO-LiPA in three samples. Conclusions: A novel Digene test is suitable for the detection of hr-HPV genotypes in clinical samples and it provides comparable results to the well established INNO-LiPA assay. Although INNO-LiPA identified significantly more samples with multiple HPV genotypes than the Digene test, the clinical benefit of such a difference is at present unclear. | |
| Descriptors | PAPILLOMAVIRUS, HUMAN GENOTYPE HYBRIDIZATION |