Avtor/Urednik		Okamoto, Shu-ichi; Krainc, Dimitri; Sherman, Katerina; Lipton, Stuart A
Naslov		Antiapoptotic role of the p38 mitogen-activated protein kinase-myocyte enhancer factor 2 transcription factor pathway during neuronal differentiation
Tip		članek
Vir		Proc Natl Acad Sci U S A
Vol. in št.		Letnik 97, št. 13
Leto izdaje		2000
Obseg		str. 7561-6
Jezik		eng
Abstrakt		Myocyte enhancer factor 2 (MEF2) is in the MADS (MCM1agamous-deficiens-serum response factor) family of transcription factors. Although MEF2 is known as a myogenic factor, the expression pattern of the MEF2 family of genes (MEF2A-D) in developing brain also suggests a role in neurogenesis. Here we show that transfection with MEF2C, the predominant form in mammalian cerebral cortex, induces a mixed neuronal/myogenic phenotype in undifferentiated P19 precursor cells. During retinoic acid-induced neurogenesis of these cells, a dominant negative form of MEF2 enhances apoptosis but does not affect cell division. The mitogen-activated protein kinase p38alpha activates MEF2C. Dominant negative p38alpha also enhances apoptotic death of differentiating neurons, but these cells can be rescued from apoptosis by coexpression of constitutively active MEF2C. These findings suggest that the p38alpha/MEF2 pathway prevents cell death during neuronal differentiation.
Deskriptorji		APOPTOSIS CELL DIFFERENTIATION DNA-BINDING PROTEINS MYOGENIC REGULATORY FACTORS NEURONS SIGNAL TRANSDUCTION TRANSCRIPTION FACTORS TRANSFECTION TUMOR CELLS, CULTURED