Avtor/Urednik		Korošec, Peter; Rijavec, Matija; Šilar, Mira; Kern, Izidor; Košnik, Mitja; Osolnik, Katarina
Naslov		Deficiency of pulmonary Valpha24 Vbeta11 natural killer T cells in corticosteroid-naive sarcoidosis patients
Tip		članek
Vir		Respir Med
Leto izdaje		2009
Jezik		eng
Abstrakt		Invariant natural killer T (NKT) cells might contribute to the amplified and prolonged T-cell immune response that characterizes sarcoidosis. Therefore, we want to investigate the frequency and distribution of pulmonary invariant NKT cells in corticosteroid-naive patients with sarcoidosis. We used multi-parameter flow cytometry with antibodies against CD3, CD4, CD8, CD14, CD19, CD45, CD16/56, TCR Valpha24, and TCR Vbeta11, on bronchoalveolar lavage fluid (BALF), to examine the frequency and distribution of pulmonary invariant NKT cells in 47 newly diagnosed sarcoidosis patients and in 8 control subjects. The frequencies of BALF Valpha24 Vbeta11 invariant NKT cells were significantly lower in patients with sarcoidosis in comparison to control subjects. Moreover, lower invariant NKT cell frequencies in patients with sarcoidosis significantly correlated with exaggerated BALF lymphocytosis and CD4 T cell responses. This study demonstrated a pulmonary deficiency in the frequency of a subset of T cells with immunoregulatory function in patients with sarcoidosis.
Deskriptorji		SARCOIDOSIS BRONCHOALVEOLAR LAVAGE FLUID FLOW CYTOMETRY KILLER CELLS, NATURAL T-LYMPHOCYTE SUBSETS CD4-POSITIVE T-LYMPHOCYTES CD8-POSITIVE T-LYMPHOCYTES RECEPTORS, IGG ANTIGENS, CD19 ANTIGENS, CD45 ANTIGENS, CD56 GENE REARRANGEMENT, ALPHA-CHAIN T-CELL ANTIGEN RECEPTOR GENE REARRANGEMENT, BETA-CHAIN T-CELL ANTIGEN RECEPTOR ADRENAL CORTEX HORMONES ROC CURVE