Author/Editor		Stojan, J; Pavlič, MR
Title		The influence of an organosulfonate on the reaction between Ellman's reagent and thiocholine
Type		članek
Source		J Anal Toxicol
Vol. and No.		Letnik 18, št. 3
Publication year		1994
Volume		str. 165-7
Language		eng
Abstract		The aim of this work was to elucidate the effect of methanesulfonyl fluoride on the detection reaction for the determination of cholinesterase activity. The effect of methanesulfonyl fluoride was studied by monitoring the time course of the appearance and disappearance of the detection reaction product using a stopped-flow technique. The obtained experimental data were analyzed by progress curve analysis. It was found that the effect of methanesulfonyl fluoride results from a reaction between methanesulfonyl fluoride and the detection reaction product with the following rate constant: k1 = 5.33 x 10(-2) M-1s-1. In evaluating the effect of methanesulfonyl fluoride, the decomposition of this agent in water was included in the analysis because the decomposition considerably affected the reaction between methanesulfonyl fluoride and the product. The corresponding rate constant was found to be k2 = 4.9 x 10(-5) M-1s-1.
Descriptors		CHOLINESTERASE INHIBITORS DITHIONITROBENZOIC ACID SULFONES THIOCHOLINE CHOLINESTERASES