Avtor/Urednik		Goličnik, Marko
Naslov		Estimation of kinetic parameters for enzyme-Inhibition reaction models using direct time-dependent equations for reactant concentrations
Tip		članek
Vir		Acta Chim Slov
Vol. in št.		Letnik 59, št. 1
Leto izdaje		2012
Obseg		str. 207-11
Jezik		eng
Abstrakt		To facilitate the determination of a reaction type and its kinetics constants for reversible inhibitors of Michaelis-Mententype enzymes using progress-curve analysis, I present here an explicit equation for direct curve fitting to full time-course data of inhibited enzyme-catalyzed reactions. This algebraic expression involves certain elementary functions where their values are readily available using any standard nonlinear regression program. Hence this allows easy analysis of experimentally observed kinetics without any data conversion prior to fitting. Its implementation gives correct parameter estimates that are in very good agreement with results obtained using both the numerically integrated Michaelis-Menten rate equation or its exact closed-form solution which is expressed in terms of the Lambert W function.
Deskriptorji		ENZYME INHIBITORS CATALYSIS KINETICS TIME FACTORS NONLINEAR DYNAMICS MODELS, THEORETICAL