Avtor/Urednik		Swenson, Luke C.; Poljak, Mario; Tomažič, Janez
Naslov		Comparative performances of HIV-1 RNA load assays at low viral load levels
Tip		članek
Vol. in št.		Letnik 52, št. 2
Leto izdaje		2014
Obseg		str. 517-523
ISSN		0095-1137 - Journal of clinical microbiology
Jezik		eng
Abstrakt		Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of %100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia.
Proste vsebinske oznake		HIV-1 RNA load levels antiretroviral therapy HIV-1 RNA stopnje obremenitve protivirusna terapija