Avtor/Urednik		Compan, Vincent; Baroja-Mazo, Alberto; Bragg, Laricia; Verkhratsky, Alexei; Perroy, Julie; Pelegrin, Pablo
Naslov		A genetically encoded IL-1[beta] bioluminescence resonance energy transfer sensor to monitor inflammasome activity
Tip		članek
Vol. in št.		Letnik 189, št. 5
Leto izdaje		2012
Obseg		str. 2131-2137
ISSN		0022-1767 - Journal of immunology (Baltimore, Md. : 1950)
Jezik		eng
Abstrakt		Inflammation is fundamental for protecting the organism against infection and injury. However, a failure to control immune response results in chronic inflammation and several associated disorders such as pain and loss of function. Initiation of inflammation is orchestrated by cytokines, among which IL-1beta is particularly important. IL-1beta is synthesized as an inactive protein that has to be processed by the inflammasome to generate the mature bioactive form. Conventional techniques cannot monitor IL-1beta activation with high spatial and temporal resolution. In this study, we present a ratiometric biosensor that allows monitoring IL-1beta processing in real time, with a temporal resolution of seconds and with a single-cell spatial resolution. Using this sensor, to our knowledge, we describe for the first time the kinetic of the inflammasome activity in living macrophages. With this new probe, we also demonstrated that the pro-IL-1beta processing occurs all over the cytoplasm.
Proste vsebinske oznake		inflammation immune response IL-beta cytoplasm