Avtor/Urednik		Berlon, Nicholas R.; Qi, Robert; Sharma-Kuinkel, Batu K.; Joo, Hwang-Soo; Park, Lawrence P.; George, Dennis; Thaden, Joshua T.; Messina, Julia A.; Maskarinec, Stacey A.; Müller-Premru, Manica; Lejko-Zupanc, Tatjana
Naslov		Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins
Tip		članek
Vol. in št.		Letnik 71, št. 4
Leto izdaje		2015
Obseg		str. 447-457
ISSN		0163-4453 - The Journal of infection
Jezik		eng
Abstrakt		Background: Phenol-soluble modulins (PSMs) are amphipathic, pro-inflammatory proteins secreted by most Staphylococcus aureus isolates. This study tested the hypothesis that in vitro PSM production levels are associated with specific clinical phenotypes. Methods: 177 methicillin-resistant S. aureus (MRSA) isolates from infective endocarditis (IE), skin and soft tissue infection (SSTI), and hospital-acquired/ventilator-associated pneumonia (HAP) were matched by geographic origin, then genotyped using spa-typing. In vitro PSM production was measured by high performance liquid chromatography/mass spectrometry. Statistical analysis was performed using Chi-squared or Kruskal-Wallis tests as appropriate. Results: Spa type 1 was significantly more common in SSTI isolates (62.7% SSTI; 1.7% IE; 16.9% HAP; p < 0.0001) while HAP and IE isolates were more commonly spa type 2 (0% SSTI; 37.3% IE; 40.7% HAP; p < 0.0001). USA300 isolates produced the highest levels of PSMs in vitro. SSTI isolates produced significantly higher quantities of PSM[alpha]1-4, PSM[beta]1, and [delta]-toxin than other isolates (p < 0.001). These findings persisted when USA300 isolates were excluded from analysis. Conclusions: Increased in vitro production of PSMs is associated with an SSTI clinical source. This significant association persisted after exclusion of USA300 genotype isolates from analysis, suggesting that PSMs play a particularly important role in the pathogenesis of SSTI as compared to other infection types.
Proste vsebinske oznake		MRSA skin and soft tissue infection pneumonia MRSA okužba kože in mehkih tkiv plučnica