| Author/Editor | Žerovnik, E; Kos, J; Lenarčič, B; Turk, V | |
| Title | Refolding of chicken cystatin and human stefin A studied by fast protein liquid chromatography (FPLC) | |
| Type | članek | |
| Source | Biol Chem Hoppe Seyler | |
| Vol. and No. | Letnik 371, št. Suppl | |
| Publication year | 1990 | |
| Volume | str. 151-6 | |
| Language | eng | |
| Abstract | Denaturant gradient gel-chromatography ŠEndo, S. et al. (1983) Anal. Biochem. 131, 108-120Ć has been used to follow renaturation of chicken cystatin and human stefin A. Slow gradient of GuHCl was applied to the size-exclusion Superose 12 column. The column was maintained at 5 degrees C by circulating water from a cryostate. When the denatured protein has been injected it started to refold on the column. By the simplified equation, according to ŠHanai, R. et al. (1986) Biophys. Chem. 25, 27-36Ć approximate kinetics of folding was obtained. No intermediate species in folding could be detected from 6M to 2M GuHCl, only the close-to-denatured state and the close-to-native state. | |
| Descriptors | CYSTATINS CHICKENS CHROMATOGRAPHY, GEL CHROMATOGRAPHY, HIGH PRESSURE LIQUID KINETICS PROTEIN CONFORMATION PROTEIN DENATURATION TEMPERATURE |