Avtor/Urednik		Batson, Sarah; De Chiara, Cesira; Majce, Vita; Lloyd, Adrian J.; Gobec, Stanislav; Rea, Dean; Fülöp, Vilmos; Thoroughgood, Christopher W.; Simmons, Katie J.; Dowson, Christopher G.; Fishwick, Colin W.G.; de Carvalho, Luiz Pedro S.; Roper, David I.
Naslov		Inhibition of D-Ala
Tip		članek
Vol. in št.		, št. Vol. 8
Leto izdaje		2017
Obseg		str. 1-7
ISSN		2041-1723 - Nature communications
Jezik		eng
Abstrakt		D-cycloserine is an antibiotic which targets sequential bacterial cell wall peptidoglycan biosynthesis enzymes: alanine racemase and D-alanine:D-alanine ligase. By a combination of structural, chemical and mechanistic studies here we show that the inhibition of D-alanine:D-alanine ligase by the antibiotic D-cycloserine proceeds via a distinct phosphorylated form of the drug. This mechanistic insight reveals a bimodal mechanism of action for a single antibiotic on different enzyme targets and has significance for the design of future inhibitor molecules based on this chemical structure.
Deskriptorji		Farmacevtska kemija Kemoterapija
Proste vsebinske oznake		D-cycloserine D-alanine ligase peptidoglycan biosynthesis enzymes nuclear physics experimental particle physics protons Compton scattering