Author/Editor		Milek, Miha; Wyler, Emanuel; Landthaler, Markus
Title		Transcriptome-wide analysis of protein-RNA interactions using high-throughput sequencing
Type		članek
Vol. and No.		Letnik 23, št. 2
Publication year		2012
Volume		str. 206-212
ISSN		1084-9521 - Seminars in cell & developmental biology
Language		eng
Abstract		Protein-RNA interactions are emerging as an important functional element in the regulation of gene expression. Cross-linking of proteins to RNA by UV irradiation followed by immunoprecipitation (CLIP) has provided a crucial tool for research in this field. Initially, the bottleneck of the method was the relatively low number of identified RNA binding sites. It was only the arrival of next-generation sequencing that allowed a comprehensive and unbiased description of the cross-linked protein-RNA fragments. Here, we summarize recent progress in the study of protein-RNA interactions, as well as some of the important findings obtained using different CLIP approaches in cultured cells and organisms. These efforts allowed the identification of functional RNA-binding sites for a wide range of RNA-interacting proteins. Experimental and bioinformatic progress will further advance this dynamic area of research. The combination of high-resolution protein-RNA interaction maps with transcriptome-wide data describing the stability, modifications and structures of RNAs, in addition to protein expression profiling, will provide deeper insight into post-transcriptional and translational regulatory events and mechanisms.
Keywords		post-transcriptional regulation protein-RNA interactions cross-linking and immunoprecipitation post-transkripcijska regulacija interakcije beljakovin in RNA povezovanje in imunoprecipitacija