Avtor/Urednik		Tratnjek, Larisa; Kreft, Marko; Kristan, Katja; Erdani-Kreft, Mateja
Naslov		Ciliary beat frequency ofin vitrohuman nasal epithelium measured with thesimple high-speed microscopy is applicable for safety studies of nasal drugformulations
Tip		članek
Vol. in št.		, št. Vol. 66
Leto izdaje		2020
Obseg		str. 1-9
ISSN		1879-3177 - Toxicology in vitro : an international journal published in association with BIBRA
Jezik		eng
Abstrakt		Nasal drug formulations can be effective for local delivery of therapeutic drugs to the sinonasal mucosa or for systemic drug delivery by absorption directly into the bloodstream. The growing field of potential nasal therapies includes nasal vaccination and even treatment of neurodegenerative diseases. However, it is important that nasal drug formulations don't have a disruptive effect on the cilia and mucosa of nasal epithelium. Mucociliary clearance represents the first host defence of the respiratory tract that requires the coordinated beating of cilia. A key parameter to determine mucociliary clearance is ciliary beat frequency (CBF). The objective of this study was to validate the high-speed digital imaging for CBF measurements in nasal MucilAir in vitro model and to test its potential for ciliotoxicity studies to evaluate the safety of investigational nasal drug formulations. Our CBF measuring setup was first validated by benzalkonium chloride, a common-practice preservative with cilio-inhibiting effect. Next, MucilAir model was treated with mometasone nasal spray (Mommox/Mometasone Sandoz). Short term cilio-stimulatory effect and dose dependent effect of mometasone nasal spray were demonstrated. Post-treatment analysis showed un-altered ultrastructure of MucilAir model. In conclusion, characterization of the ciliary activity of nasal MucilAir in vitro model and its response to relevant agents with herein developed efficient and reproducible set up for CBF analysis show great potential of this model for airway ciliotoxicity studies.
Proste vsebinske oznake		nasal drug formulations mucociliary clearance ciliary beat frequency formulacije nosnih zdravil mukociliarni očistek ciliarna frekvenca utripa