Avtor/Urednik		Zemljič Jokhadar, Špela; Stojković, Biljana; Vidak, Marko; Sorčan, Tjaša; Liović, Mirjana; Gouveia, Marcos; Travasso, Rui; Derganc, Jure
Naslov		Cortical stiffness of keratinocytes measured by lateral indentation with optical tweezers
Tip		članek
Vol. in št.		Letnik 15, št. 12
Leto izdaje		2020
Obseg		str. 1-13
ISSN		1932-6203 - PloS one
Jezik		eng
Abstrakt		Keratin intermediate filaments are the principal structural element of epithelial cells. Their importance in providing bulk cellular stiffness is well recognized, but their role in the mechanics of cell cortex is less understood. In this study, we therefore compared the cortical stiffness of three keratinocyte lines: primary wild type cells (NHEK2), immortalized wild type cells (NEB1) and immortalized mutant cells (KEB7). The cortical stiffness was measured by lateral indentation of cells with AOD-steered optical tweezers without employing any moving mechanical elements. The method was validated on fixed cells and Cytochalasin-D treated cells to ensure that the observed variations in stiffness within a single cell line were not a consequence of low measurement precision. The measurements of the cortical stiffness showed that primary wild type cells were significantly stiffer than immortalized wild type cells, which was also detected in previous studies of bulk elasticity. In addition, a small difference between the mutant and the wild type cells was detected, showing that mutation of keratin impacts also the cell cortex. Thus, our results indicate that the role of keratins in cortical stiffness is not negligible and call for further investigation of the mechanical interactions between keratins and elements of the cell cortex.
Proste vsebinske oznake		epitelijske celice kortikalna togost optična pinceta epithelial cells cortical stiffness optical tweezers