Author/Editor		Poljak, M; Orlowska, J; Cerar, A
Title		Human papillomavirus infection in esophageal squamous cell papillomas: a study of 29 lesions
Type		članek
Source		Anticancer Res
Vol. and No.		Letnik 15
Publication year		1995
Volume		str. 965-70
Language		eng
Abstract		The etiology and pathogenesis of esophageal squamous cell papillomas (ESCP), rare benign tumors of human esophagus, are still controversial. Chronic musocal irritation and infection with human papillomaviruses (HPV) are two proposed etiologies. To investigate these hypotheses, we screened 29 ESCPs from 28 patients originating from Slovenia and Poland for HPV infection using in situ hybridization (ISH) and polymerase chain reaction (PCR). No evidence of HPV DNA was found using ISH. By PCR, the presence of HPV DNA was detected in only one lesion using two different HPV L1 concensus primer sets. The restriction fragment analyses of PCR product showed patterns unique to HPV type 6. All other ESCPs were successfully amplified only with internal control human beta-globin primers. Our results show that HPV DNA is not frequently detectable in ESCPs, even when highly sensitive methods like PCR are used and that other pathogenetic mechanisms are more important in the etiology of ESCPs.
Descriptors		PAPILLOMAVIRUS, HUMAN PAPILLOMA ESOPHAGEAL NEOPLASMS POLYMERASE CHAIN REACTION IN SITU HYBRIDIZATION