Author/Editor		Jezernik, K; Medalia, O; Aronson, M
Title		A comparative study of the desquamation of urothelial cells during gestation and in adults mice following moderate stress or endotoxin treatment
Type		članek
Source		Cell Biol Int
Vol. and No.		Letnik 19, št. 11
Publication year		1995
Volume		str. 887-93
Language		eng
Abstract		The present paper deals with the compartive EM study of the detachment of mouse urinary bladder epithelial cells under various physiological conditions, namely, during gestation and in adult mice following induction by endotoxin or by exposure to moderate stress. It has been shown that desquamation during gestation involves two distinct modes, shedding of single cells and formation of apoptotic bodies. Moderate stress in adult female mice induced by constant illumination for 72 or 96 hours, results in desquamation of superfical and intermediate cells. Application of LPS is followed by desquamation of single cells and whole sheets of cells. Cell detachment involves interruption of tight junctions between neighbouring cells and formation of numerous cup shaped vesicles, multivesicular bodies and vacuoles at the base of desquamating cells. LPS induces desquamation entails delivery of lysosomal enzymes extracellulary into the intercellular space. In the areas of sloughing the bladder epithelium lack permeability barrier. Desquamated cells in the bladder lumen are mostly alive. These results clearly demonstrate the existence of specific adhesion mechanisms of detachment and desquamation of uroepithelial cells.
Descriptors		BLADDER ENDOTOXINS KERATOLYTIC AGENTS STRESS CELL ADHESION GESTATIONAL AGE INCLUSION BODIES EPITHELIUM LIGHT LIPOPOLYSACCHARIDES PREGNANCY MICROSCOPY, ELECTRON, SCANNING MICE, INBRED STRAINS APOPTOSIS