Avtor/Urednik		Zidar, N; Gale, N; Coer, A; Kambič, V
Naslov		Expression of Ki-67 antigen and proliferative cell nuclear antigen in benign and malignant epithelial lesions of the larynx
Tip		članek
Vir		J Laryngol Otol
Vol. in št.		Letnik 110, št. 5
Leto izdaje		1996
Obseg		str. 440-5
Jezik		eng
Abstrakt		In an attempt to analyse the proliferative activity in benign ang malignant laryngeal epithelial lesions, and to determine the relationship to their histologic grade, we studied the expression of proliferative cell nuclear antigen (PCNA) and Ki-67 angiten on 20 swuamous cardinomas, and on 30 biopsies of epithelial hyperplasia categorized according to the Kambič-Lenart classification into cimple, abnormal, and atypical hyperplasia. In simple hyperplasia, both antibodies stained the nuclei of the occasional cells in the basal layer. In abormal hyperplasia (mild dysplasia), positive cells occupied up to a third, and in atypical hyperplasia (moderate and severe dysplasia) they occupied from two-thirds to the entire epithelial thickness. In squamous carcinoma, we found a statistically significant correlation between its grade and the percentage of Ki-67-(p less th 0.01) and PCNA-(p less th. 0.00001) positive cells. Our results suggest that the proliferative fraction progressively increases with the degree of epithelial hyperplasia and the grade of cariconoma. We conclude that the patterns of immunoreactivity to PCNA and Ki-67 antigen correpsond to the histologic grade of both benign and malignant epithelial lesions of the larynx. This method should be regarded as a useful adjunct to traditional histological tecniques allowing more objective grading of benign and malignant epithelial lesions.
Deskriptorji		LARYNGEAL NEOPLASMS PROLIFERATING CELL NUCLEAR ANTIGEN ANTIGENS, NEOPLASMS HYPERPLASIA IMMUNOHISTOCHEMISTRY BIOPSY CARCINOMA, SQUAMOUS CELL NEOPLASM STAGING