Avtor/Urednik		Šinigoj-Gačnik, Ksenija; Doganoc, Darinka Zdenka
Naslov		Določanje olakvindoksa v prašičjem mišičnem tkivu s HPLC in UV detekcijo
Prevedeni naslov		Detection of olaquindox in porcine muscle tissue with HPLC and UV protection
Tip		članek
Vir		Zb Vet Fak Univ Ljublj
Vol. in št.		Letnik 34, št. 1
Leto izdaje		1997
Obseg		str. 15-21
Jezik		slo
Abstrakt		Olaquindox, one of derviates of quinoxaline-di-N-oxides, is used as a feed additive in stockraising, especially in pig husbandry. Since it is considered to be a genotoxic agent, its use as a veterinary drugs is temporarily accepted. Various methods were developed for the determination of olaquindox in the tissues of pigs. Our work was based on the procedure of Nagata and Saeki š(8)Ć but introducing a few modifications. The porcine muscle tissue is homogenized in acetronitrile, the extract is evaporated to dryness and redissolved in acetonitrile-water (95:5). Further purification is done on alumina basic prepacked columns. The eluat is concentrated by evaporation under nitrogen and residues are finally redissolved in methanol. Quantification is done using C18 reversed phase column with acetonitrile-water (10:90) as mobile is 0,03mg/kg of olaquindox in porcine muscle tissue. The recovery of olaquindox ranged from 54% to 79% with the coefficient of variation of 7% at 0,1 and 0,2 mg/kg and 18% at 0,05 mg/kg. Standard curves using blank muscle tissue fortified at 0,05 to 0,2 mg/kg showed good linear correlation with the correlation coefficient of 0,9976 +- 0,21% measured over 5 consecutive days. In order to yield better accuracy of the results our suggestion is to construct standard curve with matrix with each series of examined samples.
Deskriptorji		MUSCLE, SKELETAL VETERINARY MEDICINE CHROMATOGRAPHY SWINE