Avtor/Urednik		Vince, Adriana; Poljak, Mario; Seme, Katja
Naslov		DNA extraction from archival Giemsa-stained bone-marrow slides: comparison of six rapid methods
Tip		članek
Vir		Br J Haematol
Vol. in št.		Letnik 101
Leto izdaje		1998
Obseg		str. 349-51
Jezik		eng
Abstrakt		The ability of six rapid DNA extraction procedures to provide DNA for the polymerase chain reaction from archival Giemsa-stained bone marrow slides was tested on 120 samples. Boiling in distilled water, freeze-thaw method., boiling in 10% Chelex-100 resin solution, proteinase K/Tween 20/NP-40 method coupled with simplified phenol/chloroform/isoamyl alcohol protocol or salting-out procedure using saturated NaCl and modification of commercial QIAamp procedure (Qiagen, Clatsworth. Calif.) gave DNA extraction efficiencies of 50%, 70%, 85%, 95%, 100% and 100%, respectively. Our results demonstrate that rough DNA extraction methods have decreased efficiencies compared to complete DNA extraction protocols and that the latter are required to ensure highly reproducible results from archival Giemsa-stained bone marrow slides.
Deskriptorji		DNA HISTOLOGICAL TECHNIQUES BONE MARROW EXAMINATION ARCHIVES AZURE STAINS POLYMERASE CHAIN REACTION BONE MARROW