Author/Editor		Kuhar, Irena; Žgur-Bertok, Darja; Grabnar, Miklavž
Title		Uravnava izražanja gena za aktivnost kolicina K
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 304-7
Language		slo
Abstract		Colins are bacteriocins synthesized by a large number of Escherichia coli isolates. The role and mode of action of pore-forming colicins are very similar. The regulation of expression of structural genes of different colicins could be therefore quite similar, too. On the other hand strains can produce more than one colicin and cell use individual regulatory proteins in different ways to regulate their production. In the present report we used a cka-lacZ fusion and beta-galactosidase activity was measured to determine cka expression during growth. Our results indicate that expression of gene cka increases in the stationary phase due to depletion of nutrients and that ppGpp is a positive effector of the cka gene expression.
Descriptors		ESCHERICHIA COLI COLICINS GENE EXPRESSION REGULATION, BACTERIAL