Avtor/Urednik		Jurič, R; Dragaš, AZ; Seme, K; Ružić-Sabljić, E; Grošelj, D
Naslov		Vpliv parodontalne bolezni na diagnostične postopke pri limski boreliozi
Prevedeni naslov		The influence of periodontal disease on diagnostic procedures in Lyme disease
Tip		članek
Vir		Zobozdrav Vestn
Vol. in št.		Letnik 53, št. 3-4
Leto izdaje		1998
Obseg		str. 81-7
Jezik		slo
Abstrakt		The aim of this study was to estimated the cross-reactivity between Borrelia burgdorferi sensu lato, the bacterium responsible for Lyme disease, and Treponema denticola, one of the most common oral spirochaetes isolated from subgingival dental plaque in patients with moderate to advanced periodontitis. T. denticola ATCC 35404 was cultivated anaerobically in NOS medium. The treponemas were used as whole-cell antigens in the indirect fluorescent-antibody (IFA) staining procedure, with which the reactivity between T. denticola and IgM and/or IgG antibodies against B. burgdorferi sensu lato was assessed. Serum samples from 303 persons suspected of having Lyme disease were tested. The control group consisted of sera obtained from 50 patients with no history of any spirochaetal disease. In the study, we optimised the conditions for conditions for routine cultivation of T. denticola in NOS medium in our laboratory. We found a statistically significant positive correlation between the values of IgM and IgG antibodies against B. burgdorferi sensu lato and T. denticola (IgM: r = 0.253, p < 0.005; IgG: r = 0.692, p < 0.001). The results of our IFA studies indicate a significant positive correlation between B. burgdorferi sensu lato and T. denticola ATCC 35404 (p < 0.005). Since our study demonstrated a significant cross-reactivity between B. burgdorferi sensu lato and T. denticola, positive results of serological tests for Lyme disease should be interpreted with caution, especially when typical clinical signs and symptoms of Lyme disease are absent. In such cases, a periodontal status survey may help identify false positive results due to the presence of periodontal disease.
Deskriptorji		LYME DISEASE PERIODONTAL DISEASES BORRELIA BURGDORFERI FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT TREPONEMA DIAGNOSTIC ERRORS CROSS REACTIONS