Author/Editor		Drobnič-Košorok, M; Zrimšek, P; Zdovc, I
Title		Immunogenic proteins in dermatophyte mycelial extracts
Type		članek
Source		Acta Dermatovenerol Alp Pannon Adriat
Vol. and No.		Letnik 7, št. 3-4
Publication year		1998
Volume		str. 129-33
Language		eng
Abstract		Background. Dermatophyte cytosolic proteins and cell wall proteins as well different metabolites secreted by fungi are known to be involved in eliciting humoral and cellular immune response in invaded host. Methods. SDS-PAGE and Western blot techniques were used to study the imunogenic nature of the cytosolic proteins extracted from two dermatophyte species Microsporum canis and Trichophyton mentagrophytes and two non dermatophyte fungal species Malassezia pachydermatis and Aspergillus fumigatus. Crossreactivity was confirmed with ELISA technique. Results. SDS-PAGE analysis of cytosolic proteins revealed several protein bands, which are common to the dermatophytes examined, together with some distinctive bands, which enabled us to discriminate between the dermatophyte species. However, comparison of the cytosolic protein profile obtained from dermatophytes and nondermatophyte fungi studied demonstrated a much higher extent of species-specific proteins. The crossreactivity nature of the investigated fungal antigens was observed in ELISA, and developed for the detection of specific IgG in cats infected with Microsporum canis. Conclusion. Besides strongly crossreacting antigenic protein bands, Western blot analysis, using the same serum samples as in ELISA, also disclosed species-specific immunogenic proteins, which may be the candidates for further investigation of cellular immune response and development o protective vaccines.
Descriptors		DERMATOPHYTES FUNGAL PROTEINS CATS MICROSPORUM ENZYME-LINKED IMMUNOSORBENT ASSAY BLOTTING, WESTERN TRICHOPHYTON MALASSEZIA ASPERGILLUS FUMIGATUS ELECTROPHORESIS, POLYACRYLAMIDE GEL